Year: 2017 | Month: August | Volume 7 | Issue 4
Absence of NsiI Polymorphism in Growth Hormone Receptor (GHR) Gene in Indian Cattle Breeds
Abstract:
The objective of the present study was to investigate the polymorphism in 5′ non-coding region of growth hormone receptor (GHR) gene in Sahiwal (n = 53) and Hariana (n = 50) cattle using NsiI/PCR-RFLP assay. Amplification of DNA sample revealed 302bp product using specific primer pairs and digested by using NsiI restriction enzyme. All the screened animals were found monomorphic in nature for GHR/NsiI polymorphism. It revealed that only one type of uncut banding pattern (AA genotype); which was of 302bp. We could not identify any animal with GG and AG genotypes. Consequently, we could not perform association studies with milk production traits.
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